![]() Improved Dual Color Standards are brighter than the competition on both gels and blots. Increased overall brightness provides visual robustness throughout the entire protein electrophoresis and western blotting workflow. protein was detected in wild-type Arabidopsis plants by western blot assay at 14 to 28 days post-inoculation (dpi) but not in CP transgenic plants (Figure. 8 blue-stained bands and 2 pink reference bands (25 and 75 kD).Protein electrophoresis and Western blotting. ![]() Mixture of 10 recombinant proteins of precise molecular weights (10–250 kD) The molecular weight of proteins was determined by comparison with the prestained protein marker.Use improved Precision Plus Protein Dual Color Standards for molecular weight estimation on SDS-PAGE gels and western blots. Recommended volume per well for 1.0 mm mini gelġ0 μL for electrophoresis, 5 μL for blottingĥ μL for electrophoresis, 2.Place this handy selection guide magnet on your refrigerator or lab bench to eliminate the guesswork and make protein standard selection simple. Protein ladders or molecular weight markers are among the most commonly used reagents in biochemistry experiments. Life Science Group Bulletin 5561 Rev E US/EG 12-1881 12 Bio-Rad Laboratories, Inc. For more information, refer to bulletin 5792. Precision Plus Protein WesternC Standards are combination protein standards containing ten prestained, Strep-tagged recombinant proteins, enabling both fluorescent visualization on gels and. ~160, 120, 80, 60, 50, 40, 30, 20, 15, 10 kDaĮach protein standard contains a 6X His-tag enabling the detection with the InVision His-tag In-gel StainĬolorimetric or methods that detect phosphorylated proteins such as Pro-Q Diamond phosphoprotein gel stainsĬolorimetric or methods that detect glycosylated proteins such as Pro-Q Glycoprotein stain kits Multifluorescent western blotting enables detection of two antibody-specific proteins at once, removing the variability involved with two blots or the loss of protein with stripping. Western blotting protein standards can be used for both fluorescent visualization and colormetric or chemiluminescent immunodetection on western blots. The ChemiDoc MP Imaging System is an instrument for imaging and analyzing gels and western blots. Peppermint Stick Phosphoprotein Molecular Weight StandardsĬand圜ane Glycoprotein Molecular Weight Standards Experience Bio-Rads New ChemiDoc MP - One System. Tagged- proteins in the 10 -250 kDa range contain a Strep-tag™ II sequence and can be detected on western blots using Strep-Tactin™ conjugates or an antibody against Strep-tag™ II sequenceĭirect visualization can be achieved through standard staining protocols (e.g., Coomassie, etc.) Consisting of recombinant proteins fused to an IgG binding site, these versatile molecular weight ladders bind the primary or secondary antibody used for the detection of the target protein. Tagged-proteins contain an integral Strep-tag™ II sequence and can be detected on western blots using Strep-Tactin™ conjugates or an antibody against Strep-tag™ II sequence Western blot protein ladders are designed for protein molecular weight estimation directly on the blot during detection. PageRuler Unstained Broad Range Protein LadderĪccurate estimation across a broader range Recommended Volume per well for 1.0 mm gel (μL)Ĭolorimetric, NIR fluorescence (700nm channel, blue bands), RGB fluorescence (550 nm channel, orange bands) Visible monitoring of gel separation and transfer efficiency Spectra Multicolor Broad Range Protein Ladderīest multicolor prestained ladder for routine applicationsĤ colors for improved visualization during separation and transferĪnalysis of high molecular weight proteins
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